TY - JOUR
KW - Peer Reviewed Paper
KW - Checked
AU - Elkins Mark
AU - Harbour C.
AU - Punch G.
AU - Syrmis M.
AU - Rose B.
AU - Nissen M.
AU - Sloots T.
AU - Bye P.
AB - <p>
Since the role of respiratory viruses in lung exacerbations of patients with cystic fibrosis has been hampered by the difficulty of detecting viruses in viscous sputum specimens, a multiplex reverse transcriptase PCR (RT-PCR) assay combined with colorimetric amplicon detection was tested for the identification of seven common respiratory viruses in the sputa of cystic fibrosis patients. Of 52 sputa from 38 patients, 12 (23%) samples from 12 patients were positive for a respiratory virus (4 for influenza B, 3 for parainfluenza 1, 3 for influenza A and 2 for respiratory syncytial virus). These results suggest that the RT-PCR method carried out on sputum may provide a convenient means of investigating the role of virus infection in lung exacerbations of cystic fibrosis patients.
</p>

BT - European Journal of Clinical Microbiology and Infectious Diseases
C1 - 2.330
C2 - 2.309
CN - N
DA - 129284899490
J2 - Eur J Clin Microbiol Infect Dis
LA - eng
LB - MSjournal
M1 - 1
N1 - HERDC category C1
N2 - <p>
Since the role of respiratory viruses in lung exacerbations of patients with cystic fibrosis has been hampered by the difficulty of detecting viruses in viscous sputum specimens, a multiplex reverse transcriptase PCR (RT-PCR) assay combined with colorimetric amplicon detection was tested for the identification of seven common respiratory viruses in the sputa of cystic fibrosis patients. Of 52 sputa from 38 patients, 12 (23%) samples from 12 patients were positive for a respiratory virus (4 for influenza B, 3 for parainfluenza 1, 3 for influenza A and 2 for respiratory syncytial virus). These results suggest that the RT-PCR method carried out on sputum may provide a convenient means of investigating the role of virus infection in lung exacerbations of cystic fibrosis patients.
</p>

PY - 2005
SE - 2.309
SP - 54
EP - 57. [Impact Factor 2.309]
ST - Eur. J. Clin. Microbiol. Infect. Dis.
T2 - European Journal of Clinical Microbiology and Infectious Diseases
TI - Method for detection of respiratory viruses in the sputa of patients with cystic fibrosis
VL - 24
ER -